Input box
The server takes as input a protein sequence or a list of sequences.
You can paste your sequence (or set of sequences) selecting:
- Enter UniProt accessions (you need your Uniprot accession code/s)
- Paste your sequences (FASTA format)
If a target sequence is not present in the BAR-hMG database the website starts a BLAST alignment against the clusters containing magnesium binding templates.
If the sequence falls into one of those clusters the magnesium binding sites from the corresponding template are transferred. The process takes only few seconds and when the program finishes the end user will be automatically redirected to the result page.
Output
The "human magnesome" output is provided in a table that contains the following information:
- Target sequence is the UniProtKB accession code of the target sequence. The link leads to the page relative to the sequence on the UniProtKB website (if available).
- BAR+ cluster: link to the BAR+ info page about the cluster containing the target sequence. If you want to know more about the data shown in cluster info page, please read the BAR+ help page.
- the sequence in FASTA format, with its magnesium binding sites highlighted
- Positions in the target sequence: a list of binding sites containing the name of each residue and its position.
- an explanation of the labeling colors and their meaning
- a list of the matching PDBs, linking to their relative page on the PDB website
- the number of Mg ions
- Cluster HMM: a link to the HMM of the cluster